Paper: Caged metabolic precursor for DT-diaphorase responsive cell labeling



Ruibo Wang, Kaimin Cai, Hua Wang, Chen Yin and Jianjun Cheng


In this work, we report selection of covalent linker on anomeric position of N-azidoacetylmannosamine (ManNAz) for caging its metabolic process. We synthesized DT-diaphorase responsive metabolic precursor HQ-NN-AAM using the optimized linker. The caged metabolite showed responsiveness to DT-diaphorase in vitro, resulting in metabolic incorporation of azido sugar on cell surface in multiple cell lines.