2018-04-06
Authors:
Ruibo Wang, Kaimin Cai, Hua Wang, Chen Yin and Jianjun Cheng
Abstract:
In this work, we report selection of covalent linker on anomeric position of N-azidoacetylmannosamine (ManNAz) for caging its metabolic process. We synthesized DT-diaphorase responsive metabolic precursor HQ-NN-AAM using the optimized linker. The caged metabolite showed responsiveness to DT-diaphorase in vitro, resulting in metabolic incorporation of azido sugar on cell surface in multiple cell lines.